BIO 4115 Chapter Notes - Chapter 5-6: Dna Replication, Helicase, Zygosity
12 Apr 2020
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Explain the di erent next-generation sequencing methods seen in class. Comment on how 4th-generation methods are an improvement over 3rd generation methods. 3rd generation: smrt known sequence of ssdna hairpin adapters create circular template, can be sequenced numerous times. Larger fragments of 3kbp, read sequences real time. Highly e cient dna pol immobilized at the bottom of the well. Add tons of phosopholinked hexaphosphate nts that di use in the solution, the complementary one will be incorporated. Fluorescence pulse will be recorded then it is innately cleaved (don"t need to add cleaving agent) Dna pol constantly displace dsdna and continue around the circle. 3rd gen faster b/c don"t need cleaving agent but still was based on replication and a 2nd signal. Doesn"t copy the dna, instead it reads nt sequence directly. Dna has ssdna hairpin on one end and motor protein (helicase) on the other. Protein pore that"s electrically resistent is applied a voltage which result in a current.
