Textbook Notes (363,666)
Canada (158,520)
Biology (653)
BIO153H5 (147)


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Christoph Richter

Week 1 MicroscopyObjectivesBe able to describethe proper techniques for use of the compound and dissecting microscopesthe proper use of the compound and dissecting microscopestotal magnification of a compound microscopemagnification working distance parfocal parcentric illumination depth of fieldhow to prepare a wet mountthe differences between the compound and dissecting microscopesBe able to identifythe parts of the compound and dissecting microscopes and note their functionsOverviewThe microscope remains a pivotal tool in learning and research in biology to this day Typical prokaryotic or eukaryotic cells range from 0001 mm to 005 mm in diameter with only the very largest cells 01 mm entering into the range discernible by the human eye Thus microscopes are needed for detecting the existence of most cells and for visualizing cellular components You will be using the compound light microscope and the dissecting microscope also called the stereomicroscope Effective use of these microscopes is one of the fundamental skills required for studying biology many of your TAs and professors still use these skills on a daily basis in their research so dont take them lightly and it takes some practice to master this skill This laboratory will show you how to use these instruments to their best advantage Complete the following tasks to understand how to properly use a compound microscope and a dissecting microscope You will apply these basic skills in many of the subsequent weeks so take full advantage of this first lab to become fully acquainted with these invaluable toolsThe compound microscopeActivity 11 Learning the parts of the compound microscopeMaterials compound microscopesample slideProcedureMicroscopes are expensive and delicate scientific instruments please use them with care and follow the proper handling procedure1 Ensure that your work area is clean and uncluttered 2 Identify the parts of your compound microscope shown in Figure 11 and note the functions of these parts Table 11 3 Carefully clean the glass slide oculars and objectives with lens paper only Consult your TA if there is a scratch or smudge on any of the lenses after you have cleaned them You only need a small piece of lens paper to get the job done 4 Place the sample slide on the stage and use the metal slide holder to hold it in place Centre the specimen under the objective
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