BIO130H1 Chapter Notes - Chapter 18: Molecular Cloning, Plasmid, Dnac
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BIO130H1 Full Course Notes
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Dna cloning is a technique to produce large quantities of a specific dna segment. Once the amount of dna has been sufficiently amplified, the recombinant dna can be purified and used in other procedures. Section 1: the building blocks of life: antibiotic resistance allows investigators to select for those cells that contain the recombinant plasmid. As first demonstrated by avery, macleod, and mccarty, bacterial cells can take up dna from their medium: this phenomenon forms the basis for cloning plasmids in bacterial cells. One of the great benefits of dna cloning is that, in addition to producing large quantities of particular dnas, it allows one to separate different dnas from a mixture. Because a large number of different recombinant plasmids were present initially in the medium, the different cells plated on the dish contain different foreign dna fragments. A less common cloning vector is the bacteriophage lambda.
