BIO130H1 Chapter Notes - Chapter several: Nucleosome, Interphase, Ubiquitin
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BIO130H1 Full Course Notes
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Readings (from last week): 411-414; 400-406 (skip human perspectives) Factors that affect rate of reannealing: (1) ionic strength of solution (2) incubation temp. and (3) length (4) [dna] and (5) size of dna nucleic acid hybridization. Bacterial and viral dna, symmetrical curves, reanneal at similar rates. Eukaryotic dna reanneal at different rate steps highly repeated fraction: 1-10% of dna, short (few hundred bps at most), repeated. Satellite dna: 5-few hundred, clusters of several million bps can be separated into distinct satellite band evolve quickly, can vary btw. closely related species localized around centromere. Minisatellite dna: 10 to 100 bps, clustered up to 3000 repeats unstable, differs between generations variable/polymorphic in length, used in dna fingerprinting! Genome sequencing: prokaryote: first complete sequence in 95 eukaryote: budding yeast, s. cerevisae in 96. Mouse, rat and human genomes compares 5% conserved, non-coding included therefore must have some function! Humans and chimps share 4% of genome tens of millions of differences.
