BIO130H1 Chapter Notes - Chapter 1: Cell Membrane, Mortar And Pestle, Chromatin
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BIO130H1 Full Course Notes
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Cold temperatures preserve flies by slowing down chemical reactions that would break them down. Grind flies with a pestle. (1 tube for each student pair) Homogenize for 30 sec. until all big pcs. broken. Grinding the flies helps break open the cell membranes of fly cells. Rinse debris off pestle into tube containing homogenized flies with. 125 l of sb+ into tube containing homogenized flies with. If fly tissues adhering to walls of tube above level of sb+, use yellow pipettor tip to push it into sb completely. Close tube caps tightly and label tubes. Proteinase k: enzyme that digests cell membrane proteins, release dna from nucleus (proteins are contaminating dna sample) Centrifuge tubes for 3 min. at 14000 rpm. Use p200 with yellow tip, transfer 200 l of supernatant to new 1. 5 ml centrifuge tube. Avoid disturbing debris @ bottom of tube, try to avoid debris at top as well.
