PCL102H1 Chapter Notes - Chapter 7: High-Throughput Screening, Broad Institute, Immunodeficiency
17 Feb 2017
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Video: high throughput screening in an academic setting. Incubate for 24 hours: day 2: compound plates (preparation and addition to cells) o compounds are diluted and pipetted into master plates o vprep is used to add the compounds to cells o. Incubate for 72 hours: day 5: alamar blue addition and data capture o vprep used to add alamar blue o. Incubate for 4 hours o plates are put into stackers and data is captured and later analyzed. Compound screening at the broad institute (industry setting) Pcl102 session 7: high throughput screening: completely automated in an industry setting. Targets in infectious disease, and the advantage of targets in the host compared to targets in the infectious organism. When you add molecules that target the host to the water, they rescue them better than conventional antibiotics: these molecules are less likely to generate resistance because the human doesn"t evolve as rapidly as bacteria.
