Biology 1201A Chapter Notes - Chapter 16: Genome-Wide Association Study, Intron, Archaea
24 Mar 2018
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1. genome sequence determination and annotation obtaining the sequences of complete genomes and analyzing them to locate putative protein-coding and noncoding rna genes and other functionally important sequences. 16. 2 genome sequence determination and annotation obtaining the sequence of bases in a genome using dna sequencing techniques and then analyzing the sequence data using computer based approaches to identify genes and other sequences of interest. Dna sequencing was developed in the late 1970s by maxam and gilbert and then sanger designed the current method a few years later general dna sequencing steps. Dna fragmentation amplification of fragments sequencing each fragment assembly of fragment sequences into genome sequences. Dna ligase attaches short double stranded dna adaptor molecules to each end of genomic dna fragments. 20500 protein-coding genes -> 8 exons/gene -> introns make up 95% of the transcription unit more than 20 regulatory sequences are associated with each protein-coding gene.
