Biology 1201A Chapter Notes - Chapter 15: Molecular Cloning, Restriction Enzyme, Dna Ligase
Document Summary
Genetic engineering = the use of dna technologies to alter genes for practical purposes. Biotechnology = the manipulation of living organisms to produce useful products. A clone is a line of genetically identical cells or individuals derived from a single ancestor. By similar reasoning, dna cloning is a method for producing many copies of a pirece of. The piece of dna is referred to as a gene of interest, which is a gene that a researcher wants to study or manipulate. Cloned genes and other cloned dna sequences from genomes are sued in both basic research and applied research: basic research. In basic research, a researcher might want to study a cloned gene to learn about its structure, including its dna sequence and sequences that regulate its expression: applied research. 15. 1a bacterial enzymes called restriction endonucleases form the basis of dna cloning.