BIOL 2021 Chapter Notes - Chapter 16.3: Tubulin, Colchicine, Paclitaxel

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Same nucleation process as actin except with gtp. T form adds (gtp) and d form leaves (gd) Constant energy is required to keep the structure in the same phase (gtp gdp and back) Q: taxol stabilizes microtubules and causes free tubulin to assemble into microtubules, while colchicine has the opposite effect and prevents microtubule formation. A: cell division depends on a dynamic spindle made up of microtubules, which must depolymerize and repolymerize during division. Microtubules are responsible for making spindle fibres, which pull apart chromosomes in anaphase. Gamma-tubulin plus some other proteins create a ring complex and serves as a template that creates a microtubule with 13 protofilaments. Creates a minus end and grows towards the plus end. Microtubules are attached to and radiate from an mtoc (microtubule organizing centre) The centrosome (near the nucleus) is the major mtoc in animals contains gamma-tubulin ring complexes.

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