BIOL 2905 Chapter 9.11: Methods for Evaluating Disinfectants
26 Oct 2017
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Antiseptics: higher concentrations & fresher solutions of disinfectants are more effective than dilute, older solutions, longer exposure ensures death of more organisms. Phenol coefficient: evaluates efficiency of disinfectants & antiseptics, cal(cid:272)ulates a ratio(cid:374) that co(cid:373)pares a(cid:374) age(cid:374)t"s a(cid:271)ility to (cid:272)o(cid:374)trol (cid:373)i(cid:272)ro(cid:271)es to phenol. Larger the ratio, greater the effectiveness: ratio greater than 1. 0 indicated agent is more effective than phenol, has (cid:271)ee(cid:374) repla(cid:272)ed (cid:271)y (cid:374)ewer (cid:373)ethods (cid:271)/(cid:272) disi(cid:374)fe(cid:272)ta(cid:374)ts that"re (cid:373)ore effective than phenol. Use-dilution test: metal cylinders dipped into broth cultures of bacteria at dried. Researchers immerse contaminate cylinder into dilution of disinfectant being evaluated. Cylinders removed, washed & placed into tube of sterile medium for 48 hours: most effective agent entirely prevent growth at highest dilution, current standard test in us, new standard procedure being developed. Kelsey-sykes capacity test: alternative assessment approved by european union, determines capacity of given chemical to inhbit bacterial growth, researches add bacterial suspensions to chemical being tested.
