BIOL 3051 Chapter Notes - Chapter 9.0: Nuclear Receptor, Bicalutamide, Levothyroxine
24 Apr 2020
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Look at how to identify ligand for receptors & how to figure out where receptors are binding in genome. Adopting orphans: figuring out ligand for receptor that wasn"t known before. Why study orphan nuclear receptors: to discover new signally pathways, to discover new drugs, for every nuclear receptor hormone, there"s a drug. Accutane bind to rar skin disease. Orphan receptors- unknown ligands: we may know receptor, but don"t know endogenous ligand, orphan adopted orphans, some have synthetic ligand, we don"t know endogenous ligand so its underlying physiology is unknown, discovered through molecular biology: Cloning in silico= searching genome databases & expressed sequence tags (ests) for sequences that are similar to sequences in known members of nuclear receptor superfamily (e. g. Nuclear receptor superfamily: we can use luciferase reporting assay to exam what orphan receptors do, what they respond to, partially deorphanized we don"t know if we know true endogenous ligand. Endocrinology: classical: physiological effect hormone receptor genomic info.
