BIOL 311 Chapter Notes - Chapter 6: Liposome, Scanning Electron Microscope, Somatic Cell Nuclear Transfer
19 Aug 2016
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Gfp (green flourescent protein: is an example of a new technology that can be used in different ways. We can use it as a reporter molecule (it can report back to us something that is happening in the cells). Ligand receptor binding: a naturally occurring or laboratory developed hormone. Due to a difference in fluorescence, l-r binding can be validated. It had to be between 1-10 nm distance. Cameleon molecule: example: calmodulin binds calcium as a result, there is a change in cell behavior. Other related techniques: autoradiography: use of radiography compounds for tracking cell activity. You have to use a radioactive compound which is not good. But there are some things that must use radioactive compounds: dna synthesis: you want to track it using autoradiography. Pick the appropriate radioactive compound, such a titrium: protein synthesis: probe is titrium-leucine, fish (fluorescent in situ hybridization): it can detect select genes or messenger rnas throught a process.
