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BE 4303 Chapter : BE Lab Report 1

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azuremammoth471
15 Mar 2019
School
LSU
Department
Biological Engineering
Course
BE 4303
Professor
All

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Document Summary

The purpose of this lab assignment is to create a histogram with the given data using. A histogram is a display of rectangles, called bins, whose width is equal to the class interval and whose area is representative of the frequency of the data items. Histograms are useful in representing frequencies of large data sets and presenting a summary of the data. The materials necessary for the lab are the data points, a computer, and the microsoft. To create a histogram one must first input all the given data and identify the highest and lowest values. Next, the boundaries and bin increments must be determined. The number of bins correlates to the number of bars on the chart. After determining the number of bins, in microsoft excel, go to data , and select data analysis , and then click histogram . If data analysis is not available, install it by clicking microsoft office button and selecting excel.

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Related Questions

Cell Membranes Lab Report

  1. Diffusion

  1. Compare the following terms: solvent, solute, andsolution.

  1. Go to Lab, Section I, Exercise 1 to explore the factorsinfluencing the rate of diffusion and complete the following:

  1. Predict how molecular mass and temperature may affect theoutcome of this experiment.

  1. Record the data from information in the Lab in the tablebelow.

Time

(min)

Diameter (mm)

5°C MB

5°C PP

23°CMB

23°C PP

0

15

30

45
  1. Construct a line graph of the data from the table above. Yourgraph should include labeled axes, units, and a legend indicatingwhich treatment is presented. Sign, date and prepare an image ofyour graph and include it with this lab report.
  1. Osmosis

  1. Go to Lab, Section II, Exercise 2 to view a demonstration ofosmosis and answer the following questions:

  1. Describe the net movement of water in osmometer 1.
  1. How is the movement of water molecules related to theconcentration gradient of the solution?

  1. Go to Lab, Section II, Exercise 3 to observe the effect ofsolute concentration on the rate of osmosis. Answer the followingquestions:

  1. Was the net movement of water in bags 1 to 4 into or out of thebags?
  1. Explain the results from bags 4 and 5.
  1. Selective Permeability of Membranes

  1. Go to Lab, Section III, Exercise 4 to learn about theimportance of selectively permeable membranes, then complete thetable and answer the following questions:

Contents of Beaker

0 Min

30 Min

Starch

Chloride ion
  1. Which substances diffused through the dialysis membrane?

  1. How does dialysis tubing model the selective permeability of aplasma membrane?
  1. Tonicity

  1. Go to Lab, Section IV, Exercise 5 and 6 to observe plasmolysisin Elodea cells and tonicity in red blood cells. Summarizethe concept of tonicity using blood and Elodea cells asexamples. Your description should incorporate these terms: turgid,plasmolysis, hemolysis, and crenation.

Cells

Isotonic Solution

Hypertonic Solution

Hypotonic Solution

Elodea

RBC

  1. Based on your previous work, reproduce the table below and drawan illustration of a single cell in each box. Include arrows toshow the direction of water flow relative to each cell and dots tosymbolize solutes. Sign, date and prepare an image of your drawingand include it with this lab report.

Cells

Isotonic Solution

Hypertonic Solution

Hypotonic Solution

Elodea

RBC

Summary Questions

  1. Compare diffusion and osmosis. Give an example of each.

2. In which direction will osmosisoccur if a 15% sugar solution is separated from a 25% sugarsolution by a selectively permeable membrane?

3. Why did osmosis, but not diffusionof sucrose molecules, occur across the dialysis membrane containing20% sucrose solution?

4. You are having a party and thecelery is limp. What might you do to make the celery crisp (turgid)again? What will occur in the cells?

5. A small amount of fertilizer(mineral salts) will stimulate plant growth, but over fertilizationcan kill plants? Why?

6. Michael adds sugar to his coffee.Explain what in this drink is the solvent, solute, and solution.

7. You add a cube of sugar to yourdrink. How could you speed up the diffusion of sugar moleculeswithout stirring?

8. How is dynamic equilibriumestablished within a solution?

Tube # 1% Strach pH 6.8 buffer Distilled water 0.1% amylase Time to starchdisappearance
1 0 mL 2 mL 2 mL 1 mL 30
2 0.5 mL 2 mL 1.5 mL 1 mL 300
3 1.0 mL 2 mL 1.0 mL 1 mL 360
4 1.5 mL 2 mL 0.5 mL 1 mL 8:03
5 2.0 mL 2 mL 0 mL 1 mL 11

You collected data in the form of time to starch disappearance.However, enzyme activity is usually quoted as a rate. Convert yourcollected data to rate form (mg of substrate consumed / unit time),and produce a graph of this data (rate vs. substrateconcentration): hint: you must first convert the starch volumes toamounts using the C1V1 = C2V2 equation, and convert 1% starch in tolg/ 100mL equivalent,

Use Appendix 1 for hints using EXCEL

Which quantity, rate or substrate concentration, should be onwhich axis?

What trend does your data show, and does this support or fail tosupport the hypothesis you propose in #1, above?

HELP ON ECOLOGY LAB REPORT PLEASE!!!

- The AIM of this lab exercise: Does Eastern Mosquitofish (Gambusia holbrooki) population structure differ with season?

- You will compare population structure in an FIU pond from February 2018 (previous semester) with the data that you collect this semester (September 2018).

- CREATE HYPOTHESIS ABOUT POPULATION STRUCTURE (HELP PLEASE)

(HELP PLEASE) Include at least one paragraph ON THE key points of comparison between the life tables. Include the major results for population structure, even if no difference is found! Do these populations differ with respect to optimal age of sexual maturity, R0, G, and r?

FEBRUARY 2018 CURRENT RESULTS

Age Class (days)
x		 Sample/ Age
s(x)		 Number/ Age
n(x)		 Survival Rate
1(x)		 Fecundity b(x) Offspring/Ind 1(x)*b(x)

Age Weighted Fecundity 1(x)*b(x)*x
0 24 120 1 0 0 0
30 39 96 0.8 0 0 0
60 19 57 0.475 0 0 0
90 21 38 0.317 24 7.608 684.72
120 8 17 0.142 27 3.834 460.08
150 5 9 0.075 29 2.175 326.25
180 2 4 0.033 32 1.056 190.08
210 0 2 0.017 0 0 0
240 1 2 0.017 0 0 0
Sum 120 R0

14.673 Offspring
G 113.209 Days
r

0.024 Ind/ Days

Optimal Age for Sexual Maturity

 120 Days

SEPTEMBER 2018 RESULTS:

Life Table
Age class (days) Sample/ age class Number/ age class Survival rate Fecundity Offspring/ individual Age-weighted fecundity
x n(x) n(x) l(x) b(x) l(x)*b(x) l(x)*b(x)*x
0 9 150 1 0 0 0
30 12 141 0.94 0 0 0
60 26 129 0.86 0 0 0
90 29 103 0.687 24 16.48 1483.2
120 33 74 0.493 27 13.32 1598.4
150 18 41 0.273 29 7.93 1189
180 16 23 0.153 32 4.91 883.2
210 7 7 0.047 0 0 0
240 0 0 0 0 0 0
Sum 150
R0 42.63 offspring
G 120.89 days
r 0.03 individuals/ day
Optimal age for sexual maturity 120 days