BIOL 4246 Chapter : 6 18 12 Translation
Document Summary
Transcription elongation myriad of other proteins that interact with rna pol and modulate its activity. Rna pol in vitro does not need any of them, however: Nusa- increases the length of pauses, interacts with rna pol and product and makes it more sensitive to signals. Greb- activate an activity of the rna pol allowing to remove unpaired bases from a 3" end of a transcript. Saves the rna from an arrested complex, also improves the fidelity ofrna by adding a pseudo proofreading function. Nusg- increase elongation rate along the dna in copying it, almost counter to nusa because it lengthens pauses. 5" ppp- ---rbs- ---aug/gug/uug-----orf including aug------uaa/aug/uga-----stem loop---- poly u 3" the type of termination that affects the transcript affects its stability in the cell. Rho dependent are degraded faster than rho independent because of the secondary structure of the stem loop and the dsrna makes the rho independent harder to degrade.
