BIL 150 Chapter Notes - Chapter 17: Rna Splicing, Tata Box, Spliceosome

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Transcription = dna rna: in nucleus. Translation = mrna polypeptide: in cytoplasm. A primary transcript is the initial rna transcript from any gene prior to processing. Rna polymerase pries two dna strands apart and adds nucleotides. Promoter where rna polymerase attaches and initiates transcription: terminator to end it in bacteria, extend several dozen nucleotides upstream of start point. Transcription unit is stretch of dna that is transcribed into rna. 3 stages of transcription: initiation: after rna polymerase binds, dna strand unwinds and polymerase initiates rna synthesis at start point. Lucas tozzi: elongation: moves downstream unwinding and elongating rna transcript 5" 3". In wake of transcription dna strands re-form double helix: termination: rna transcript is eventually released and polymerase detaches. Elongation: gene can be transcribed simultaneously by several rna polymerases: nucleotides added to 3" end. Modifying pre-mrna: alteration of mrna ends. 5" end gets a cap (modified form g nucleotide) 3" end gets poly-a tail (50-250 more a nucleotides)

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