BIL 255 Chapter Notes - Chapter 10: Northern Blot, Electrophoresis, Primary And Secondary Antibodies
Document Summary
The isolation and manipulation of genes allows for more precise genetic analysis as well as practical applications in medicine, agriculture, and industry: fundamental changes in our society are occurring as a result of genetic engineering. Restriction enzymes (re) are endonucleases that will recognize specific nucleotide sequences in the dna and break the dna chain at those points. A variety of re have been isolated and are commercially available. Most cut at specific palindromic sites in the dna (sequence that is the same on both antiparallel dna strands). These cuts can be a staggered which generate sticky or overhanging ends or a blunt which generate flush ends. Once you have isolated and cut the donor and vector dnas, they must be joined together. The dnas are mixed together in a tube. If both have been cut with the same re, the ends will match up because they are sticky.
