BY 108 Chapter Notes - Chapter 14: Dna Polymerase Iii Holoenzyme, Semiconservative Replication, Okazaki Fragments
Document Summary
Meselson-stahl experiment - supported watson/crick"s theories on dna replication. Semiconservative replication - each strand of dna duplex used when forming new dna. Conservative replication - original dna duplex remains intact, new. Dispersive replication - original dna gets scattered in new dna, which contains new/old molecules on each strand. Bacteria w/ heavier nitrogen (15n) isotope in dna grown and then transferred to bacteria w/ lighter nitrogen isotope (14n) Centrifuge used to determine density of dna after replication. Results showed that dna replicates in a semiconservative way replication process - must be fast/accurate. Starts at origin, goes bidirectionally towards the terminus. Replicon - functional unit containing chromosome and origin. Polymerase - enzyme that synthesizes nucleic acids. 3 main ones: pol i, pol ii, pol iii. Dna pol ii used mainly for dna repair. Dna pol iii - made up of alpha subunit (main catalytic part) and beta subunit (forms ring around template, acting as sliding clamp)
