BIOL 212 Chapter Notes - Chapter 10: Nucleic Acid Hybridization, Reverse Transcriptase, Noncoding Dna

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Dna molecule synthesized from an mrna molecule and therefore lacking the introns that are present in genomic dna cdna library. Collection of dna fragments synthesized using all the mrnas present in a particular type of cell as a template. Standard method of determining the nucleotide sequence of dna; utilizes dna. Dna library polymerase and a set of chain-terminating nucleotides. Production of many identical copies of a dna sequence. Collection of cloned dna molecules, representing either an entire genome (genomic library) or copied of the mrna produced by a cell (cdna library) Enzyme that reseals nicks that arise in the backbone of a dna molecule; in the. Gene replacement lab can be used to join together two dna fragments. Genetically engineered animal in which a specific gene has been inactivated. Technique that substitutes a mutant form of a gene for its normal counterpart to investigate the gene"s function. Collection of cloned dna molecules that represents the entire genome of a cell.

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