BIOL 212 Study Guide - Midterm Guide: Reduction Potential, Antibody, Coenzyme Q10
28 Mar 2018
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Document Summary
Isolating a gene from a chromosome: generating new (recombinant) dna molecules in a test tube. Introducing recombinant dna molecules back into a living organism. Because the central dogma allows us to store all info in the form of dna and easily convert to rna and protein. Making recombinant dna molecules nucleic acid hybridization polymerase chain reaction (pcr) Restriction enzymes are dna endonucleases, recognize specific dna sequences and perform hydrolysis on sugar-phosphate backbone, splitting/cutting specific nucleotide sequences. Once dna molecule cleaved into smaller pieces, dna fragments separated by size (length) Negatively charged dna molecules travel towards + electrode, larger fragments slower. Each band composed of dna molecules of identical length. Dna fragments from restriction enzymes to recombinant dna molecules. Dna fragments generated from restriction enzymes can be joined using dna ligase to create recombinant dna molecules. Circular double-stranded plasmid dna is the cloning vector, which is cleaved by restriction nuclease, and dna fragment inserted with dna ligase to make recombinant dna.
