BIOL1006 Lecture Notes - Lecture 13: Gel Electrophoresis, Transcriptome, Transcriptomics Technologies
9 Jun 2018
School
Department
Course
Professor
Lecture 13: biotechnology
Transcriptomes
- The set of RNA molecules produced by an organism, organ or group of cells
- Defines the genes that are being expressed
- Transcriptomics= study of
transcriptome (complete set of RNA
transcripts that are produced by the
genome under specific circumstances
or in a specific cell
Blue= under expressed relative to others
Red= over expressed
- Can see what genes are changing
Restriction enzymes= endonuclease
- Bacteria are attacked by viruses called bacteriophages
- Bacteria defend themselves against viral DNA by cutting foreign DNA with restriction
enzymes
- Eah estitio eze uts at a speifi seuee aset i the hosts genome
find more resources at oneclass.com
find more resources at oneclass.com
2
Some common restriction enzymes
- They are read as palindromes
(read same both ways)
Gel Electrophoresis
- Gel electrophoresis tank (in
here we have some agar (like a
jelly). At one end there will be
wells and in each of those, we
stick DNA)
- Apply electric current
- DNA will be attracted to positive end (they
are negatively charged)
- On one side, the same piece of DNA has
been cut with restriction enzymes
- Single piece of DNA that has been extracted out of an animal
find more resources at oneclass.com
find more resources at oneclass.com
3
- if there is a mutation, then the enzyme will not cut at that site
D + E = 973 bp (longer)- instead of having 2
bands (D and E), there would only be one band
further up at 937bp
- We can cut DNA but also join them back
together using DNA ligase
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
The set of rna molecules produced by an organism, organ or group of cells. Defines the genes that are being expressed. Transcriptomics= study of transcriptome (complete set of rna transcripts that are produced by the genome under specific circumstances or in a specific cell. Bacteria are attacked by viruses called bacteriophages. Bacteria defend themselves against viral dna by cutting foreign dna with restriction enzymes. Ea(cid:272)h (cid:396)est(cid:396)i(cid:272)tio(cid:374) e(cid:374)z(cid:455)(cid:373)e (cid:272)uts at a spe(cid:272)ifi(cid:272) se(cid:395)ue(cid:374)(cid:272)e a(cid:271)se(cid:374)t i(cid:374) the host(cid:859)s genome. Some common restriction enzymes (read same both ways) Gel electrophoresis tank (in here we have some agar (like a jelly). At one end there will be wells and in each of those, we stick dna) Dna will be attracted to positive end (they are negatively charged) On one side, the same piece of dna has been cut with restriction enzymes. Single piece of dna that has been extracted out of an animal.
