BIOL 1F25 Lecture Notes - Lecture 13: Nature Medicine, Dyskinesia, Ubiquitin

• School: Brock University
• Department: Biological Sciences
• Course: BIOL 1F25
• Professor: Nicholas D Vesprini

Document Summary

18f-l-dopa uptake (pet scan results): patient 4 uptake in grafted area (arrow) increased after surgery & reached normal levels at 36 months. uptake in all other areas decreased after surgery. Staining: applied antibody that binds to tyrosine hydroxylase (th): all cells that contain dopamine also contain th: detected antibody with chemicals that make dark stain. Grafted areas of putamen (left): heavy th staining. Non-grafted areas of putamen (right): almost th staining: top: low magnification. Grafted areas of putamen: th- immuno-reactive staining found in neurons with cell bodies, axons & other processes. Electron micrographs: th-containing cells made chemical synapses with other dopamine-containing neurons? reactive cell bodies in putamen must be (cid:498)new(cid:499) neurons traced th-immuno-reactive axons neurons. suggests that new neurons from the graft restore movement. New(cid:499) or (cid:498)old(cid:499) (cid:498)old(cid:499) dopamine-containing neurons have cell bodies in substantia nigra. Th-immuno- through multiple sections to their cell bodies to identify (cid:498)new(cid:499) neurons and (cid:498)old(cid:499) neurons.