BIOL 1F90 Lecture Notes - Lecture 11: Membrane Protein, Covalent Bond, Cytoskeleton
2 Oct 2017
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Document Summary
Integral membrane proteins permanently embedded in the membrane and cannot be released without disrupting the integrity of the membrane. Results: at 0 c the fluorescence was seen on only one portion of the fused cell, at 37 c the fluorescence was distributed throughout fused cells. Experiment by watt webb used an alternative approach: cell surface proteins labelled with red fluorescence, laser beam used to bleach a small region, cells incubated at 37 c. Bleached and unbleached regions intermixed over time, confirming lateral movement of proteins. Membrane proteins may be restricted in their lateral movement if attached to: cytoskeleton, extracellular matrix. Membrane proteins participate in many important cellular processes: these include transport, energy transduction, cell signalling, secretion, cell recognition, and cell-to-cell contact. Estimated percentage of membrane proteins is substantial: 20% 30% of all genes may encode membrane proteins. Membrane proteins are important medically: approximately 70% of medications exert effects by binding to membrane proteins.
