BIOL 2P02 Lecture : Transcription and mRNA Modification

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fuchsiahare261

18 Dec 2011

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> polymerization adds phosphate that provides (atp) energy. > at the unwinding area we find a lot of tata. > by convention a gene location class one is where a transcription starts. > 1 is the first nucleotide copied into mrna. > upstream from where the first nucleotide is. > downstream is anything after the 1 which has positive numbers (this is where transcription occurs) > up and down are relative to the number 1. > the transcript is always given 5" to 3" direction. > heterogeneous nuclear rna (hnrna) is the unprocessed mrna. > the core promoter: defines the beginning of the coding regions. > rna polymerase i : transcribes 5. 8s, 18s, 28s rrnas. > rna polymerase iii: 5s mrna and trna. Ii for protein transcription fr is responsible om m. > rna polymerase ii promoter is present at the beginning of the gene. > tata box at (minus) -25 base pairs.

Related Questions

Match the following:

1	Region which lies upstream of thetranslation start site contains the Shine Dalgarno sequence
2	Sequence added to the 3' end of thepre-mRNA which affects mRNA stability
3	Establishes the reading frame andspecifies the first amino acid of the protein chain
4	DNA sequence that the transcriptionapparatus recognizes and binds to initiate transcription
5	Expressed sequences of DNA that arenot removed during alternative splicing
6	Signals the end of translation andthe protein molecule
7	Group of three nucleotides that codefor an amino acid
8	Site begins the coding region of thegene and is located 25-30 nucleotides downstream of the TATAbox
9	Non-coding sequences of DNA thatintervene within coding regions of a gene
	10 Ribosome binding site ineukaryotes; functions in the initiation of translation and mRNAstability
11	A sequence which lies downstream ofthe coding region of the gene; determines the location of the 3'cleavage site in the pre-mRNA molecule
12	Sequence of nucleotides at the 3' endof the mRNA that is not translated into protein; affects thetranslation and stability of the mRNA molecule

A.	5' untranslated region
B.	AAUAAA consensus sequence
C.	Promoter
D.	Transcription start site
E.	3' untranslated region
F.	Introns
G.	Initiation codon (AUG)
H.	Poly(A) tail
I.	5' cap
J.	Termination codon (UAA, UGA, UAG)
K.	Exons
L.	Sense codon

ivorymacaw91

The promoter region of a gene has undergone an inversion such that the 3â² end of the initiation site is upstream of the rest of the promoter sequence. This will affect the transcription of this gene in all the following ways except

a. the determination of the coding strand.

b. the orientation of the RNA polymerase.

c. the binding of RNA polymerase to the initiation site.

d. the separation of the double-stranded DNA.

e. the nucleotide with which transcription begins.

Indicate whether eac is true for gene expression in prokaryotes,eukaryotes or both:

1. After transcription, a 3' poly- A tail and a 5' cap areaddeddto the RNA.

2. Translation of mRNA can begin before transcription iscomplete.

3. RNA polymerase binds to the promoter region to begintranscription

4. RNA polymerase can only add a robonucleotide to the 3' end ofthe growing RNA

5. The mRNA transcript is the exact complement of the gene fromwhich it was copied.

lemonyak277

BIOL 2P02 Lecture : Transcription and mRNA Modification

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