HLSC 3P97 Lecture Notes - Lecture 6: Cell-Mediated Immunity, Gram Staining, Dendritic Cell
2 Apr 2018
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Antibody-mediated mechanisms do not necessarily resolve the infection. Cell mediated immunity may be the primary antigen-clearance mechanism. *rarely find cytokines in the blood, but we can find t cells. Plating out dendritic cells and telling them not to proliferate, because we want to measure proliferation of t cells only. Only way t cells proliferate is if they do recognize the antigen. T cells, dendritic cells with antigen, add radioactive marker and then read. More counts we get from a well, more proliferation. Plate original solution and then 10-fold serial dilutions. *looking for the plate that gives between 30 and 300 colonies. Plaque - formation of a bald area on a lawn of cells, indicating viral lysis. Plaque-forming unit - virus or group of viruses that forms a plaque in a lawn of cells. Differential staining (gram staining, ziehl-neelsen or acid-fast stain (afs), giemsa, silver staining, etc. )
