KINE 2P97 Lecture Notes - Lecture 3: Phosphagen, Pyruvic Acid, Covalent Bond
8 May 2018
School
Department
Course
Professor
KINE 2P97
Muscle Physiology & Exercise Metabolism
Important
1. all substrates must be present
2. law of mass action (near equilibrium)
a. rate of near equilibrium reactions determined by the concentration of substrate
and product, can proceed in both directions
3. allosteric activation/inhibition of enzyme
4. covalent modification of enzyme
a. 3 + 4 non-euiliiu, ee otollig the ezyes
b. a ezyes atiity is alteed ieased/deeased y heial odifiatio
(usually phosphorylation)
c. o-euiliiu eatio, doest deped o pesee
d. required an enzyme to catalyze the regulation (DIFFERENCE)
Coenzymes
- a non-protein compound necessary for the functioning of an enzyme
- cannot operate or catalyze reaction without the enzyme
- reaction substrates which are re-generated
NAD+ & FAD
- important transfer of electrons
- oxidation-reduction reaction
- high-energy compounds
- traded for ATP when reduced
- L.E.O says G.E.R
o Loss of electrons is oxidation, gain of electrons is reduction
- O.I.L.R.I.G
o Oxidation is losing, reduction is gaining electrons
Iediate ATP egeeatio idepedet of aoutiet eakdo pathays
- Adenylate kinase reaction
o Aka the yokiase eatio
o Phosphocreatine (PCr) system
▪ Aka the “ATP-PC, Phosphage, ad/o eatie kiase syste
Immediate ATP Regeneration by ADP
- ATP ADP + Pi + Energy
- When ADP accumulates it is converted to ATP and AMP by myokinase (or adenylate
kinase)
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
A non-protein compound necessary for the functioning of an enzyme cannot operate or catalyze reaction without the enzyme reaction substrates which are re-generated. High-energy compounds traded for atp when reduced. L. e. o says g. e. r: loss of electrons is oxidation, gain of electrons is reduction. O. i. l. r. i. g: oxidation is losing, reduction is gaining electrons. I(cid:373)(cid:373)ediate atp (cid:396)ege(cid:374)e(cid:396)atio(cid:374) i(cid:374)depe(cid:374)de(cid:374)t of (cid:373)a(cid:272)(cid:396)o(cid:374)ut(cid:396)ie(cid:374)t (cid:271)(cid:396)eakdo(cid:449)(cid:374) (cid:858)path(cid:449)ays(cid:859) Adenylate kinase reaction: aka the (cid:858)(cid:373)yoki(cid:374)ase(cid:859) (cid:396)ea(cid:272)tio(cid:374, phosphocreatine (pcr) system, aka the atp-pc(cid:396)(cid:859), (cid:858)phosphage(cid:374)(cid:859), a(cid:374)d/o(cid:396) (cid:858)(cid:272)(cid:396)eati(cid:374)e ki(cid:374)ase(cid:859) syste(cid:373) Atp adp + pi + energy. When adp accumulates it is converted to atp and amp by myokinase (or adenylate kinase: adp + adp atp + amp, t(cid:449)o adp(cid:859)s (cid:374)eeded to generate an atp. When exercise starts adp, pi and amp accumulate. The pcr system generates atp via the creatine kinase reaction. Controlled by mass action: at rest, there is lots of atp, and the body uses it to regenerate the pcr.
