BIOL 261 Lecture Notes - Lecture 8: World Federation Of Trade Unions, T-Tail, Intron

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17 Sep 2016

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The code: see figure 9-5, pg 318 in the text book. The code involves a three letter non-overlapping block of information called a codon. Each codon codes for one amino acid (or a termination signal). The mrna is read by consecutive codons of three nucleotides each with no gaps. Protein synthesis proceeds in a stepwise fashion, by a translation complex composed of the large and small ribosomal subunits with a variety of other protein factors. Translation proceeds in a 5" to 3" direction on the mrna, the protein is synthesized from the amino terminus to the carboxyl terminus. Translation starts at an aug codon (it codes for methionine), stop is signaled by. In dna these are atg, for start, tag, taa, tga for stops. ) The step-wise synthesis of proteins by ribosomes has three distinct stages 1) initiation: elongation, and 3) termination we discuss this in detail only for prokaryotes, initiation.

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1. _________________________ is the name of the process that produces proteins using RNA molecules.

a.Transcription

b Translation

c. Replication

d. Mutagenesis

2. The peptide bonding site on the ribosome:

a. A site

b. P site

c. E site

d. D site

3. The name given to the bond formed between two amino acids.

a. Hydrogen bond

b. Double bond

c. Peptide bond

d. Negative bond

4. __________________________ is the movement of the mRNA molecule on the ribosome to open up the A-site for the new incoming amino acid.

a. Transformation

b. Mutation

c. Translocation

d. Initiation

5. What does the term semi-conservative mean.

a. Replication of RNA is semi-conservative, each new strand consists of one old strand of RNA and one new strand of RNA.

b. Replication of DNA is semi-conservative, each new strand consists of one old strand of DNA and one new strand of DNA.

c. Replication of DNA is semi-conservative, each new strand consists of new strands of nucleotides.

d. Transcription of RNA is semi conservative.

6. This molecule functions during protein synthesis to recognize the codon and bring the proper amino acid back to the ribosome for translation.

a. mRNA

b. tRNA

c. rRNA

d. DNA polymerase

7. What is meant by the base-pairing rule.

a. Bases on a single strand of DNA are always found as pairs

b. Adenine always base pairs with guanine; cytosine with thymine

c. Adenine always base pairs with thymine; cytosine with guanine

d. Bases on a single strand of RNA are found in pair.

8. In this step of protein synthesis, amino acids are added to a growing polypeptide chain.

a. Initiation.

b. Elongation.

c. Termination.

d. Transpiration.

9. Three complementary bases found in a mRNA are called ____________________.

a. Anti-codon.

b. Triplet.

c. Doublet.

d. Codons.

10. Which statement best describes the job or function of the messenger RNA.

a. Carries the map of the gene sequence in the form of codons to the cytoplasm

for translation of the protein.

b. It transfers amino acids to the ribosome for protein synthesis.

c. It forms the ribosomes.

d. It helps replicate DNA.

ceruleangnat663

Fill in the blank. Elongation during translation does NOT involve ____________.

Question 16 options:

	the translation of codons according to the genetic code
	the formation of bonds catalyzed by the ribosome
	complementary base pairing between RNA molecules
	amino acids being linked together in a polypeptide
	reading the DNA template 3' to 5'

For a given gene, what establishes the reading frame for translation?

Question 17 options:

	the location of the enhancer relative to the gene
	the first three nucleotides at the 5' end of the mRNA
	the first three nucleotides at the 3' end of the mRNA
	the start codon in the mRNA
	the location of the promoter relative to the gene

Which of the following is the LEAST likely direct consequence of a substitution mutation?

Question 18 options:

	changing the length of a protein coded for by a gene
	changing one amino acid in a protein
	creating a stop codon
	eliminating a start codon
	changing the length of the DNA molecule containing a gene

Suppose that the pre-mRNA transcript from a eukaryotic gene is 30,000 nucleotides long, and the gene codes for a sequence of 300 amino acids. What is the best explanation for the relationship between these numbers?

Question 19 options:

	only the first 900 nucleotides of the pre-mRNA transcript are translated
	it takes 100 nucleotides to specify a single amino acid
	300 of the nucleotides in the transcript are important, and the rest are "junk"
	only the last 900 nucleotides of the pre-mRNA transcript are translated
	large portions of pre-mRNA transcripts are cut out during RNA processing

Suppose an individual is born into a population with a novel mutation. Is the new mutation an evolutionary change, and why?

Question 20 options:

	no, because it is not a big enough change to count
	yes, because new mutations are always adaptive
	yes, because the appearance of a new genetic variant is a genetic change in a population
	no, because not enough individuals have the mutation for it to matter
	no, because most mutations are not adaptive

RNA has many different roles in gene expression. Check all thatapply.

mRNA defines the polypeptide to be formed

DNA defines the polypeptide to be formed

rRNA, along with proteins, forms the ribosome

mRNA delivers the appropriate amino acid to the ribosome

tRNA delivers the appropriate amino acid to the ribosome

Translation is appropriately named because the process transfersthe code from DNA to RNA, though in complementary fashion.

True

False

The template strand is to the sense strand as the coding strandis to the antisense strand.

True

False

The phenomenon symbolized by DNA â RNA â Protein is referred toas the Central Dogma because it is true for all organisms.

True

False

The reading frame refers to the selection of the correct tripletof nucleotides by selecting the correct beginning point. If thebeginning point is wrong, all nucleotides will be clustered intothe wrong codons.

True

False

Activating enzymes need to recognize only one anticodon.

True

False

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BIOL 261 Lecture Notes - Lecture 8: World Federation Of Trade Unions, T-Tail, Intron

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