BIOL 461 Lecture Notes - Lecture 14: Sister Chromatids, Wild Type, Mitosis
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Biol 461 lecture 14 ** recorder stopped early. Mosaic analysis is similar to normal analysis looking at effects (phenotypes) of mutants, (cid:271)ut (cid:449)e"(cid:448)e (cid:271)ee(cid:374) looking at the entire organism. Mosaic has wt and mutant tissue. Or (cid:862)(cid:272)lo(cid:374)al a(cid:374)al(cid:455)sis(cid:863): ge(cid:374)eratio(cid:374) of (cid:862)(cid:373)osai(cid:272)(cid:863) i(cid:374)di(cid:448)iduals (cid:449)ith (cid:272)ells/tissues/stru(cid:272)tures are genetically distinct: clones can be wt (except for their marker: the mutation or gfp used to identify the clone. Developmental decisions as clonal restrictions: when and how are cells determined tocontribute to specific regions of developed organism: compartments are revealed by clonal restrictions. I(cid:374) de(cid:448)elop(cid:373)e(cid:374)t, (cid:862)a (cid:272)o(cid:373)part(cid:373)e(cid:374)t is a(cid:374) area of the de(cid:448)elopi(cid:374)g or (cid:373)ature fl(cid:455) that is (cid:272)o(cid:374)stru(cid:272)ted by all the descendants of a founding set of (cid:272)ells(cid:863) (cid:894)p. la(cid:449)re(cid:374)(cid:272)e(cid:895) Marking cells early in development before they became distinguishable from their neighbors. Genetically mark that one cell so that it expresses the marker phenotype so that you can.
