BIOL 467 Lecture Notes - Lecture 7: Histone H1, Primase, Histone H2B
Document Summary
Expose cells to a labeled brdu and then to fix the cells and look at the location of the label. Origin firing is influenced by association of chromosomes with structural components of the nucleus: replication is concentrated at discrete foci, and the location changes during s phase (i. e. late foci in heterochromatin at periphery nuleus) Depending on when the cells exposed to the label and how long = idea of where replication was occurring because of the incorporation of the brdu at the specific locations. Green pattern = brdu label, changing with time starts out central, starts to spread, and have mass spread over most of nucleus. At the very end the green dots around the periphery of nucleus. This kind of information = some influence on structure of nucleus could influence where replication is able to occur. Nuclei of mammalian cells incubated with brdu for 10 mins, incorporated onto newly synthesized dna.
