BIOL 2020 Lecture Notes - Lecture 6: Protein Kinase C, Protein Kinase A, Adenylyl Cyclase

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magentatortoise545

31 Aug 2016

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Document Summary

Cell signaling and signal transduction: communication between cells. Signal transduction - how a signal gets transmitted in a cell. Need a receptor - only talk about transmembrane receptors. Amplification of signal can cause a big reaction in the cell (cascade: activates target protein, activation of certain activities. Pathway using receptor kirosin kinase: transmembrane receptor, phosphorylation of the receptor of the itself becomes a recruiting center for certain types of proteins, activates target protein, activation of certain activities. G-protein coupled receptor is always coupled with a hetero-trimeric g-protein which is always coupled with an effector. Loops on the outside (amino group) are used to recognize the ligand - very variable. Loops on inside (carboxyl end) - less variable and used to recognize the g-protein. G-protein = alpha subunit and gamma-beta complex subunit attach to the membrane. Inactive g protein gef (guanine nucleotide-exchange factors) stimulate the dissociation of the bound gdp on the alpha subunit.

Related Questions

QUESTION 1

Which of the following is an example of an extracellular signal molecule?

	a.	IP₃
	b.	cAMP
	c.	Insulin
	d.	Diacylglycerol

2 points

QUESTION 2

Nuclear hormone receptors _____

	a.	Bind to water soluble ligands
	b.	Activate second messengers
	c.	Regulate transcription in response to ligand binding
	d.	Are normally located on the plasma membrane

2 points

QUESTION 3

Which of the following does not occur when a receptor tyrosine kinase is bound by ligand?

	a.	Phosphorylation of the RTK
	b.	Cytoplasmic proteins bind the phosphorylated RTK
	c.	Dimerization of the RTK
	d.	The RTK binds to DNA to regulate transcription

2 points

QUESTION 4

Which of these is a logical signal-transduction pathway?

	a.	An intracellular receptor activates phospholipase C, which cleaves a membrane protein to form IP₃, which then activates the opening of an ER channel protein, which releases cyclic AMP into the cytoplasm, where it binds to an intracellular enzyme that carries out a response.
	b.	A G-protein-linked receptor activates G protein, which activates phospholipase C, which cleaves a membrane lipid to form IP₃, which binds to a calcium channel on the ER, which opens to release calcium ions into the cytoplasm, which bind to an intracellular enzyme that carries out a response.
	c.	An ion-channel receptor opens, allowing a steroid hormone to enter the cell; the steroid hormone then activates protein kinases that convert GTP to GDP, which binds to an intracellular enzyme that carries out a response.
	d.	A tyrosine-kinase receptor activates adenylyl cyclase, which activates phospholipase C, which converts ATP into cyclic AMP, which binds to an intracellular enzyme that carries out a response.

2 points

QUESTION 5

Which of the following is an example of signal amplification?

	a.	activation of an enzyme molecule
	b.	activation of a specific gene by a growth factor
	c.	Breakdown of many cAMP molecules
	d.	activation of 100 molecules by a single signal binding event

2 points

QUESTION 6

Consider a signal transduction pathway that utilizes cAMP as a second messenger to activate PKA (Protein Kinase A). Which of the following situations will result in the cellular response?

		The G protein releases GDP and binds to GTP
		The target protein (adenylyl cyclase) is inactive
		The regulatory subunits of PKA (Protein Kinase A) are bound to the catalytic subunits
		The appropriate G protein is bound to GDP

2 points

QUESTION 7

Which of the following is found in only some signal transduction pathways? [In other words, which is not found in all signal transduction pathways?]

		Receptor protein
		Extracellular signal molecule
		Intracellular signaling molecule
		Cellular response
		Effector protein

2 points

QUESTION 8

The enzyme that specifically catalyzes the conversion of ATP to cAMP is:

		Adenylyl phosphatase
		Adenylyl kinase
		Adenylyl dehydrogenase
		Adenylyl cyclase

2 points

QUESTION 9

If an animal cell suddenly lost the ability to produce GTP, what might happen to its signaling system?

		It would use ATP instead of GTP to activate and inactivate the G protein on the cytoplasmic side of the plasma membrane.
		It would not be able to activate and inactivate the G protein on the cytoplasmic side of the plasma membrane.
		It would employ a transduction pathway directly from an external messenger.
		It would be able to carry out reception and transduction but would not be able to respond to a signal.

2 points

QUESTION 10

A hormone signal is sent from the brain to other parts of the body via the bloodstream. Why do only certain cells respond to this signal?

		Hormones do not last long enough in the bloodstream to stimulate a cellular response
		Only cells that have a specific transcription factor gene will be able to respond to the signal
		Only cells in the immediate vicinity of the cell sending the signal will respond
		Only cells that have a receptor for the specific signal will be able to respond to it

cerisehedgehog846

1. What types of biological macromolecules are most cell walls and extracellular matrices composed of? (Choose ALL correct answers)

[]

proteins

[]	nucleic acids

[]

lipids

[]	carbohydrates

2. What is a "second messenger"?

a.	a protein that phosphorylates another substrate

b.	a molecule that is produced or released intracellularly as a result of receptor activation

c.	a signaling molecule that is released extracellularly

GTP

3. How can the activation of a protein kinase be important in signal transduction?

a.	kinases bind to receptors to prevent the signal from binding the receptor

b.	kinases prevent signal amplification by ensuring other proteins are not active

c.	kinases can activate or deactivate existing proteins by phosphorylation

d.	kinases are secreted by the cell as a signaling molecule

limeplatypus677

A model showing a hypothesized signaling pathway for shmoo formation in yeast Fus3 kinase and formin proteins are generally distributed evenly throughout a yeast cell.

. Based on the model in the diagram, why does the shmoo projection emerge on the same side of the cell that bound the mating factor?

The only formin molecules that get phosphorylated and thus activated are those near the G protein-coupled receptor that binds mating factor.

All of the Fus3 kinase and formin molecules in the cell become activated by binding of mating factor, but the actin strands form only on the side toward the potential mate.

The G protein-coupled mating factor receptors are located on only one side of the cell, and that is where the signal transduction occurs to initiate shmoo formation.

BIOL 2020 Lecture Notes - Lecture 6: Protein Kinase C, Protein Kinase A, Adenylyl Cyclase

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