BIOL 2030 Lecture Notes - Lecture 4: Genomic Library, Nitrocellulose, Cloning

• School: Dalhousie University
• Department: Biology
• Course: BIOL 2030
• Professor: Wright Jonathan

Document Summary

Requires a mutant strand of the bacterium or host organism. The mutaion gene is the one that you are looking at cloning. Ater creaing colonies would transfer them to nitrocellulose. Then break the cells open with naoh to see the dna inside. Apply the hybridizaion probe and then wash of the excess. Expose to x-ray ilm to see which colonies have the dna. Some viruses do it using an enzymes called reverse transcriptase (it is an rna dependent. Dna polymerase needs rna as a template but makes dna) A sequence of t"s will allow dna polymerase to start and rnase will come by and digest all the rna let behind.