BIOL 200 Lecture Notes - Lecture 15: Proteinase K, Dna Barcoding, Qiagen
20 Oct 2017
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Lecture 15 - practical guide to dna barcoding: dna isolation, selecting a dna protocol, qiagen dna mini-prep kit (very good quality dna but at a cost of per sample) Squish prep (very good quality at a cost of only 5 per sample). We use this method since it is inexpensive. Ingredients: water, 5 m nacl, 0. 5 m edta, 1 m tris-hcl ph 8. 0, 20 mg/ml proteinase k (digests almost all proteins except itself and is the key component of squish prep, selecting an organism. Fly: 5 l of the master mix, add 45 l of the master mix to the fly in the 5 l (this is one of the 10 samples, close the micro test tube. Incubation: insert the micro test tube into the incubator and set the incubation method to. The(cid:374) it"s set at (cid:1006)(cid:1007) c to (cid:272)ool the tu(cid:271)e so that it (cid:272)a(cid:374) (cid:271)e ha(cid:374)dled: pcr.
