BIOL 200 Lecture Notes - Lecture 3: Helicase, Transcription Factor Ii D, Rna Polymerase Ii
7 Jun 2016
School
Department
Course
Professor
Document Summary
You can dissolve the activities of rna polymerases by their reaction to alpha amanitin: each different polymerase has a different result, use a transcription assay. If you get a signal you have something you can work with. Rna polymerase comes in 3 fractions: different abilities to be resistant to alpha amanitin, pol 1 = resistant to alpha amanitin, pol 3 = intermediately resistant, pol 2 = extremely resistant. Three eukaryotic rna polymerases: rna polymerase i. Transcribes only precursor ribosomal rna (rrna: rna polymerase ii. Transcribes messanger rnas (mrnas) and four small nuclear. Rnas (snrna) that take part in rna splicing. Transcribes transfer rna (trna), 5s rrna, and other small stable rnas including one involved in rna splicing. Protein element that can recognize a dna element. Rna pol 2 has a ctd protein tail that hangs off the complex: recruits things needed later on for elongation and stabilization.
