BIOL 201 Lecture Notes - Lecture 18: Immunoglobulin Light Chain, Microtubule
Document Summary
E. g. frog skin cells have pigment containing organelles melanosomes, can disperse or aggregate to change skin color: lab observation using squid neuron axon, organelles moving on microtubules are observed as moving dark spots two-way traffic. Post transcriptional modification of tubulins marks different roads. Kinesin: walks towards the plus end of tubules, heads are atpase, stalk coiled coil that connect heads (through linkers) with tail (or the light chain, tail kinesis receptor, select cargo, kinesin movement. Atp hydrolysis is coupled with conformational change similar mechanism as myosin head. An empty atp site has high affinity of tubule; adp-motor has low affinity of tubule. Atpase domains communicate with each other, always have one binding on the tubules, the other one switches around and put itself forward. First foot (forward motor) bind, releases adp. Forward motor head binds atp, conformational change in neck linker, causes the rear head to swing forward. New forward motor head releases adp, binds tubule.
