BIOL 202 Lecture Notes - Lecture 25: Permease, Repressor, Proteome
Document Summary
Orthologs: similar genes in different species with same function, derive a ancestral chromosome, eg mouse chromosome and human chromosome, same gene but different alighnment in the chromosome, chromosome rearrange during evolution. Functional genomic: can analysis vary gene products, transcriptome (rna transcripts) Microarray analysis (dna chip) complementary probes extract mrna and make cdna transcripts computer calculates relative levels of hybridizing (gene expression) qualitative and quantitative indication. Rna seq generate cdna library with add linker uses ngs shows the quantity of rna at a given point of time: proteome (protein products) E. g. in yeast tow-hybrid vector experiment, transcription factor dimer consists of protein parts from 2 different plasmid dna, reconstitution of the transcription factor and activation of the reporter (transcribed gene) evidence for transcription. Uses antibody that binds to target protein and purify tells which protein can bind a specific dna region: studying cancer. Knock out of several genes finding mutation that is specific to a disease lecture 26.
