BIOL 202 Lecture Notes - Lecture 18: Ap Site, Thymine, Complementary Dna
5 Nov 2015
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3 base pair insertion: can disrupt one or two codons, but no amino acid insertion/substitution, doesn"t cause frame shift, everything downstream is still the same. Ethyl group promotes pairing with thymine instead of cytosine: nitrosoguanidine (ng) - adds methyl groups, alkylation of o-6 position of g changes basepairing specificity, causing g-c --> How do we know if something is a mutagen: ames test (1974, ames noted correlation between cancer and mutations, wanted to measure mutagenicity of compounds in bacteria, but not all carcinogens are mutagens. Strain 2 = 2 his- frameshift mutant: centrifuges it and looks for revertants. February 13th, 2015: non-bulky damage, replacement of bases that don"t cause a disruption in the morphology or structure of. Dna helix: dna glycosylase cleaves base-sugar bond, ap endonuclease makes cut, drpase removes stretch of dna, polymerase synthesizes new dna, ligase seals nick.
