BIOL 300 Lecture Notes - Lecture 19: Long-Term Memory, Karyopherin, Retrovirus
5 Mar 2018
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It checks to see if there are any splicing factors still bound to the mrna. If yes the mrna is kept inside. The intron will be spliced out: then the mrna will get exported out of the nucleus, and you will get white colonies. Since the whole artificial intron has been spliced out, there is a huge chunk of sequence missing: when translated, it will give a short non-functional protein. B-galactosidase is not activated and you get white c: 2. Mlp2 and it is the unspliced version of the mrna that get exported you get blue colonies. Directed transport on cytoskeleton: you transport the mrna along the cytoskeleton (such as the microtubules: 2. Random diffusion and trapping: the mrnas randomly diffuse but then are trapped at a specific location in the cell and they become enriched: 3. Generalized degradation in combination with local protection: the mrnas are quickly degraded in the cytoplasm expect in specific areas where they are protected.
