BIOL 202 Lecture Notes - Lecture 19: Rna Interference, Lysis, Genome Editing
5 Nov 2015
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Why maize: chromosomes visible under light microscope, easy to set up crosses - male tassels, female flowers, each kernel is an embryo derived from a different egg, kernels have many scorable phenotypes. Unexpected outcome of crossing 2 colour strains: a colour gene is dominant, dark purple ears of corn in heterozygous or homozygous dominant allele, yellow corn when homozygous recessive. The activator (ac), which mcclinctock was unable to map o. In other strains, a c/c phenotype reverted to c/c. Mcclintock proposed that ac and ds were mobile. Dissociation locus inserted and disrupted dominant gene making it non- functional allowing recessive allele to be expressed. In the presence of ac, ds would mobilize out of the dominant allele, restoring its function and creating patches of purple: ds or ac insertion in c locus causes loss of function. C gene in wild type state produces pigmented kernel. When disrupted by ds - get coloured phenotype.
