BIOL 300 Lecture Notes - Lecture 16: Plasmid, Genome Editing, Protein Folding

• School: McGill University
• Department: Biology
• Course: BIOL 300
• Professor: Frieder Schoeck

Document Summary

October 18th: techniques that enable scientists to specifically modify the genetic information (genome) of divers living organisms, eg : deletion, insertions, mutations ect , reverse genetics. 1: reca in bacteria or rad51 in eukaryotes then catalyzes invasion of one of these 3 ends into the homologous region of the homologous chromosome, as described above for repair of a collapsed replication fork (step. 2: the 3 end of the invad- ing dna strand is then extended by a dna polymerase, displacing the parent strand as an enlarging single-stranded loop of dna (dark blue) (step. 3: when the loop extends to a sequence that is complementary to the other broken and 5 -exonuclease . ), the complementary sequences base- digested end of dna (the fragment on the left following step. 3: this 3 end is then extended by a dna polymerase using the displaced single-stranded loop of parent. 4: next the new 3 ends are ligated (step.