BIOL 300 Lecture Notes - Lecture 16: Zinc Finger, Cas9, Protein Folding

• School: McGill University
• Department: Biology
• Course: BIOL 300
• Professor: Frieder Schoeck

Document Summary

Genome engineering (editing: techniques that enables scientists to specifically modify the genetic information (genome) of divers living organisms, ex: deletion, insertion, mutation, etc, provides tools to do reverse genetics to target specific genes (instead of mutating randomly) If the disrupted gene is essential, these spores will be nonviable: caveat: it is not efficient in many other species such as drosophila and plants. What if dsdna breaks could be specifically introduced to a gene of interest : repair by nhej may produce insertion or deletion of few nucleotides that may disrupt the gene function, delete important amino acid, cause frame shift. Fok1 to act as dimer and cleave the dna. In zinc-finger nuclease, the zinc finger domain recognizes 3 bases however in this case each repeat recognizes a single base pair: provides a greater flexibility than zinc finger and easier to program.