BIOL 300 Lecture Notes - Lecture 20: Stem-Loop, Stop Codon, Internal Ribosome Entry Site

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Injected cdna and genomic dna and they check how much protein they get in the cytoplasm. They used the same gene and injected both types of dna. The cdna is transcribed in mrna, which is exported and eventually made into proteins. When using genomic dna, it is transcribed into pre-rna, then splicing happens and the protein is exported. In this case, we get a lot more proteins. But when we compare the mrna originating from the cdna and the genomic dna in the cyctoplasm (where the translation takes place) > identical! Because nfx nft bind to ref, which sits down only on spliced rnas. Only discussed in class that it could have been a possible explanation. The study found that the mrna was exported fine to the cytoplasm. The experimental control is important in that it tells us if the fractionation between the nucleus and the cytoplasm is functional.

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