BIOL 300 Lecture Notes - Lecture 30: Scaffold Protein, Restricted Product, Paxillin
16 Dec 2017
School
Department
Course
Professor
Document Summary
Drosophila eye mutants are viable, and we can easily screen for the mutants (they are obvious) Each ommotidium is made of 8 photoreceptor cells r1-r8. Boss : bride of sevenless, expressed on r8. Antibody staining > we can see 7 photoreceptors, because r8 is stacked under the r9. Boss or sev mutant > we can see 6 photoreceptors. > basically reduces the pathway a lot : the components could be upstream or downstream because we don"t have a dominant mutation. Is a inducing b, and b passing on the signal to c and ect: the only way to do epistasis analysis is to use dominant negative mutants. And how does it avoid a signalling mess: the cell/tissue-specific developmental history ensures that only a subset of these proteins is expressed, scaffold proteins can separate common kinases. Even though the singling is fast, there is restricted product release and no amplification. Compared to gpcrs, this pathway is not prone to amplification.
