BIOL 300 Lecture Notes - Lecture 3: Tata Box, Nucleic Acid Thermodynamics, Helicase

• School: McGill University
• Department: Biology
• Course: BIOL 300
• Professor: Frieder Schoeck

Document Summary

Lecture 3 eukaryotic rna polymerase and formation of the pol ii pre-initiation complex (pic) Unique aspects of gene organization and expression in eukaryotes: 1. In higher eukaryotes, much of the dna is non-coding, both within (intronic) and between genes: 3. Extensive processing of mrna takes place in eukaryotes: 5" cap formation, splicing, polyadenylation, 4. Nuclear transport of mrna must take place before translation can occur: 5. Amanitin this means that the three fractions contained different types of rna polymerases that had different sensitivities to -amanitin. Clamp domain of rpb1: three channels are present in rna pol ii, when they compared the free form of rna polymerase ii and the one that is actively transcribing, there was one clear difference at the clamp domain . Clamp domain rotates and closes when 8-9 nts rna-dna hybrid is present: closed clamp is recognized by elongation factors such as dsif, ensures that once the rna pol starts transcribing, it becomes stable (and the.