ANAT 262 Lecture Notes - Lecture 2: Antonie Van Leeuwenhoek, Confocal Microscopy, Microscopy

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Lecture 2: light microscopy i mercredi 13 janvier 2016. How we know what we do about cells: (1) microscopy (light and electron), (2) biochemical techniques, (3) genetic techniques (particularly in yeast) and (4) combinations of the above. Light microscopy was the most important until 1950s since other techniques were not as developed. Electron microscopy was dominant from the 1950"s to the 1970"s. A combination of biochemistry and yeast genetics dominated from the 1980"s to present. But light microscopy is making a comeback as a dominant technique due to new ability to follow dynamics of proteins in living cells. Antony van leeuwenhoek: used an apparatus with single lens (powerful magnifying glasses) mounted in a tiny hole in the brass plate that makes up the body of the instrument. The specimen was mounted on the sharp point that sticks up in front of the lens, and its positions and focus could be adjusted by turning two screws.

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