ANAT 262 Lecture Notes - Lecture 3: Stress Granule, Pex5, Xpo1
30 Aug 2016
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Document Summary
Hiv mrna has introns in later stages of life cycle, introns bound to splicing factors that have. Rev protein has nes, recognized by crm 1, allows message to be exported. Culture cells producing virus, added lmb virus not active but cells dead. Need crm1 to export other components essential for cell function (e. g. ribosomal subunits, rrna) Normal mrna: need helicases to export; helicase reorganizes rna. Crm1-dependent rna export (for hiv mrna): do not need ddp5; ddx3 instead. Ddx3 encoded by host, not made by virus. Interfere with ddx3 helicase (from host) to prevent export of virus rna if ddx3 made by host, probably has another function in cell. If want to prevent part of virus life cycle, should target components made by virus rather than host (could kill host) e. g. make dominant negative mutants of rev protein to interfere w/process. Importin alpha & beta subunits of nls receptor that move cargo into nucleus.
