BIOL 112 Lecture Notes - Lecture 12: Polymerase Chain Reaction, Cystic Fibrosis, Restriction Fragment Length Polymorphism

• School: McGill University
• Department: Biology (Sci)
• Course: BIOL 112
• Professor: Lucy- Ann Joseph

Document Summary

To make an organism express a new phenotype. To understand how the gene itself works (in isolation) -->type ii : positional cloning technology - genetic engineering to study gene in isolation this will be helpful when we don"t know what"s wrong with the disease . To discover the biochemical basis of an inherited disease, cystic fibrosis. Respiratory infections which result from an inability to move mucus out of the lungs. Before modern treatment, only treated the symptoms not the underlying cause. It"s said that midwives knew that if an infant"s sweat was excessively salty, it would die young. Sturtevant and his genetic mapping might allow us to identify and clone a gene without knowing anyhing else about it except it"s phenotype and its map position. If we find two genes that flank the cf gene and code for genes we know, then somewhere in the dna between those genes is the cf gene.