BIOL 200 Lecture Notes - Alternative Splicing, Cleavage And Polyadenylation Specificity Factor, Polyadenylation
Document Summary
Linking recruitment factors and cleaving for enzymatic activity. Elongation of poly a tail and at a faster rate, as well as when to stop adding a residues. Dna sequence rich has a poly (a) signal rich in au repeats does not get transcribed. Poly a signal (gu signal) if mutated, doesn"t work. Sequences are conserved, if mutated rna cannot be processed. Conserve and protect rna from endonucleases and mark it as an rna major step between binding complexes to sequence: is the recruitment of pap which extends repeats. Pap has to bind between aauaaa and g/u before it can cleave. Pap adds poly(a) really slowly, cleaves rna at 3" end. Regulation of splicing of specific pre-mrnas can increase genetic diversity. Control, only expressed in females in early embryogenesis. One gene regulated by multiple promoters early and late promoter for sex lethal gene auto-regulatory mechanism. Splicing regulators can act as repressors by blocking splicing or as activators.
