BIOC 300D1 Lecture Notes - Lecture 2: Enzyme, Transmittance, Absorbance

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Lecture 2: spectroscopy and enzyme kinetics. Transmittance is the fraction of light passed through an object. 0 (all of the light is absorbed) to 1 (all of the light is transmitted) Absorbance is the amount of light absorbed by an object. Absorbance is measured as the negative log of transmittance (t) Abs (or o. d) = - log10 (t) A = * c * d. This is denoted by the beer- lambert. = molar extinction coe cient (mol- 1 * l * cm- 1 ) c= molar concentration (mol/l) d= path length (cm) Complex solutions between colored and non- colored species. Particles shield each other from light at high concentration. Particles can absorb, transmit, or scatter light. @ 600 nm = 5* 108 bacteria/ml. In what other situations would the beer- lambert. If you are measuring the spectra of a mixture, what modi cations should be made to the beer- lambert.

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