BIOC 311 Lecture 17: Oxidative Phosphorylation

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Cannot have a difference in ph or membrane potential: the imm is impermeable to ions such as h+, oh-, k+, cl- whose diffusion would discharge an electrochemical gradient. Ions such as h+ can only get across the imm via specific transport proteins or channels: electron transport is associated with h+ transport out of intact mitochondria. This creates a measurable electrochemical gradient across the imm, a membrane potential, m. Convention: when an ion is transported from positive (outside) to negative (inside), is negative: reconstitution studies: reconstituted membrane vesicles containing bacteriorhodopsin, a light-driven proton pump, and atp synthase from mitochondria. When the vesicles were illuminated, atp was synthesized. The experiment clearly showed that the respiratory chain and atp synthase are biochemically separate systems linked only by a proton motive force. Oct. 14, 2015: uncoupling agents: compounds such as fccp and dnp increase the permeability of imm to protons and dissipate the proton gradient.

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