BIOCHEM 3H03 Lecture Notes - Lecture 16: Insulin-Like Growth Factor 1, Immunoassay, Abdominal Pain
12 Apr 2017
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Document Summary
Med lab technologist is reviewing results before verification and comes across an unusual result for a thyroid screen on an out-patient that has come in for pre-op blood work. He consults w/ the clinical biochemist as to whether he should report the extremely high tsh result. If tsh is normal, you are fine, not a thyroid problem. Free t4 is then measured to see any issues in the case of too high or too low. The clinical biochemist reviews the tsh protocol. What type of assay is the tsh test: discuss the investigation to determine if the result should be reported. Heterogeneous, non-competitive tsh assay: heterogeneous- requires separation w/ washing, non-competitive- utilization of a secondary ab, binds to the specimen (tsh), that are attached onto abs of coated well -> (elisa) Possibly a hama bridging interference: e. g. person has been exposed to mouse blood that has mouse abs, results in abnormally high tsh result.
