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Lecture

Prokaryotes DNA Replication Initiation

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Department
Biology
Course
BIOLOGY 2D03
Professor
Laura Parker
Semester
Fall

Description
Prokaryotes DNA Replication Initiation: protein assembly at origin: oriC, A-T rich • Helicase – separate ds • Topoisomerase • Primase – 10bp RNA primer Elongation: 5’-3’ • Okazaki  5’-3’exonuclease: RNaseH Termination: ter region termination region  pausing at fork • Topoisomerase  deconcatanate Transcription -operon -promoter: Pribnow box -10, -35  sigma factor recognize specific promoter  RNA Pol I Initiation: • Promoter binding--> open complex formation -10 to +2 ternary complex: DNA, Pol, RNA • Abortive initiation • Make 10bp sigma fall off foot print shrink 30bp Elongation • Pausing • Driven by hydrolysis of PPi • More error prone than replication • Bridge helix  Termination • Regulated by attenuation  not make unused stuff • Rho independent: GC hairping  puase, Utail (AT = less stable interaction) • rho dependent: bind rut (rho utilization)  hydrolysis of ATP to pull of Translation - 70s: 50S + 30S - mRNA polycistronic  shine delgarno  ribosome binding seq (multiple sites)  have 5’and 3’ UTR Initiation: • Shine Delgarno 6-8 poly purine  • IF1 + IF3  block other site of small subunit  Psite open • mRNA bind Shine Delgarno, • fMet -tRNa bind P site at AUG • IF2 (GTPase)  hydr
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